dna library construction vector  (TaKaRa)

Journal: Applied and Environmental Microbiology

Article Title: An Amidase Gene, ipaH , Is Responsible for the Initial Step in the Iprodione Degradation Pathway of Paenarthrobacter sp. Strain YJN-5

doi: 10.1128/AEM.01150-18

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: pUC118 BamHI/BAP , DNA library construction vector, Amp r , TaKaRa.

Techniques: Plasmid Preparation, Mutagenesis, Expressing, Gene Knockout

Journal:

Article Title: Prospecting for Novel Biocatalysts in a Soil Metagenome

doi: 10.1128/AEM.69.10.6235-6242.2003

Figure Lengend Snippet: Microbiological materials

Article Snippet: The medium contained 1.5% agar and 0.2% glucose as combined carbon sources. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Material Relevant trait(s) Source or reference E. coli strains VCS257 DP50 derivative Stratagene DH5α Cloning strain, recA1 endA1 gyrA96 thi1 relA1 hsdR17 supE44 lac Gibco-BRL BL21(DE3) Expression host for pET vectors Novagene Plasmids and cosmids pBSK+ pBluescriptSK+ multicopy cloning vector, Amp r Stratagene pTZ19R High-copy-number cloning vector, Cm r Amersham, Pharmacia pWE15 Cosmid cloning vector for construction of DNA libraries, Amp r Stratagene pIIIE5 pWE15 containing a 20.7-kb insert with possible agarase genes from microbial consortium This study pXE1 pWE15 containing a 25-kb insert with possible lipase genes from microbial consortium This study pVIIC10 pWE15 containing a 34.7-kb insert with possible agarase genes from microbial consortium This study pXB11 pWE15 containing a ca.

Techniques: Clone Assay, Expressing, Plasmid Preparation

Journal:

Article Title: Prospecting for Novel Biocatalysts in a Soil Metagenome

doi: 10.1128/AEM.69.10.6235-6242.2003

Figure Lengend Snippet: ORFs and genes identified for the sequenced contigs of cosmid clones pVIIC10, pXIVA3, pIIIE5, pXB11, pXE1, and pIIIF2 a

Article Snippet: The medium contained 1.5% agar and 0.2% glucose as combined carbon sources. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Material Relevant trait(s) Source or reference E. coli strains VCS257 DP50 derivative Stratagene DH5α Cloning strain, recA1 endA1 gyrA96 thi1 relA1 hsdR17 supE44 lac Gibco-BRL BL21(DE3) Expression host for pET vectors Novagene Plasmids and cosmids pBSK+ pBluescriptSK+ multicopy cloning vector, Amp r Stratagene pTZ19R High-copy-number cloning vector, Cm r Amersham, Pharmacia pWE15 Cosmid cloning vector for construction of DNA libraries, Amp r Stratagene pIIIE5 pWE15 containing a 20.7-kb insert with possible agarase genes from microbial consortium This study pXE1 pWE15 containing a 25-kb insert with possible lipase genes from microbial consortium This study pVIIC10 pWE15 containing a 34.7-kb insert with possible agarase genes from microbial consortium This study pXB11 pWE15 containing a ca.

Techniques: Clone Assay, Binding Assay

Journal:

Article Title: Prospecting for Novel Biocatalysts in a Soil Metagenome

doi: 10.1128/AEM.69.10.6235-6242.2003

Figure Lengend Snippet: Physical maps of the central parts of five of the different cosmids isolated in this work. The arrows indicate the locations and the directions of transcription of the ORFs on the different cosmids (pVIIC10, pXIVA3, pIIIE5, pXB11, pXE1, and pIIIF2). ORFs encoding potential agarases are indicated by red arrows. Identical agarase genes are indicated by the same letters (a to f) in parentheses; the agarases designated e and d differ in their N-terminal domains but are identical at their C termini. Possible genes with potential value in biotechnology are shown in color. Possible functions of the indicated ORFs are shown in Table ​Table2,2, together with the GenBank accession numbers. A conserved agarolytic gene cluster is enclosed in a box. ORFs uvs072, uvs093, and uvs085 in this conserved cluster code for the same 785-amino-acid protein and are indicated by gray arrows; contig ends are indicated by two slashes, and the vertical arrows indicate the positions of the two 31-bp inverted repeats. Additional data, including the results of similarity searches and the lengths of the predicted ORFs, are available on the web at http://www.gwdg.de/∼biofilm/genemining.htm.

Article Snippet: The medium contained 1.5% agar and 0.2% glucose as combined carbon sources. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Material Relevant trait(s) Source or reference E. coli strains VCS257 DP50 derivative Stratagene DH5α Cloning strain, recA1 endA1 gyrA96 thi1 relA1 hsdR17 supE44 lac Gibco-BRL BL21(DE3) Expression host for pET vectors Novagene Plasmids and cosmids pBSK+ pBluescriptSK+ multicopy cloning vector, Amp r Stratagene pTZ19R High-copy-number cloning vector, Cm r Amersham, Pharmacia pWE15 Cosmid cloning vector for construction of DNA libraries, Amp r Stratagene pIIIE5 pWE15 containing a 20.7-kb insert with possible agarase genes from microbial consortium This study pXE1 pWE15 containing a 25-kb insert with possible lipase genes from microbial consortium This study pVIIC10 pWE15 containing a 34.7-kb insert with possible agarase genes from microbial consortium This study pXB11 pWE15 containing a ca.

Techniques: Isolation